Device
OmpA-HIVcl
Part:BBa_K743011
Designed by: Bryon Silva Group: iGEM12_UC_Chile (2012-09-22)
OmpA + HIV protease cleavage site secretion system
This device is designed for the further incorporation of any protein coding sequence downstream the HIV cleavage site. Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease.
If any polypeptide sequence is fused to the 3' end of the HIV cleavage site DNA sequence, it will protrude from the outer membrane where it will be cleaved from the ompA domain by HIV protease.
This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP and a spider silk monomer.
You can check the results HERE LINK!
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
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